The Indian Journal of Veterinary Science http://ijvs.info/index.php/ijvs Pashupati Foundation India en-US The Indian Journal of Veterinary Science 2347-4025 Manifestations of Brucellosis: A Systematic Review http://ijvs.info/index.php/ijvs/article/view/33 <p><em>Brucellosis is a major bacterial zoonosis of global importance. The causative organism is Gram-negative facultative intracellular pathogens that may affect a range of different mammals including man, cattle, sheep, goats, swine, rodents and marine mammals. Brucellosis is caused by different species of Brucella and is cause significant economically losses to livestock industries. The disease is manifested by reproductive disorder, viz., infertility, retained placenta, abortions and endometritis in livestock species, hence it leads to loss in productivity of animals and consequently to economic losses. Despite the advances made in the diagnosis and therapy, brucellosis is still wide spread and its prevalence in many developing countries is increasing.</em></p> S. N. Ghodasara H. H. Savsani V. A. Kalaria A. R. Bhadaniya ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2014-06-01 2014-06-01 2 2 1 13 Application of Information and Communication Technology (ICT) in Livestock Sector- a Review http://ijvs.info/index.php/ijvs/article/view/34 <p><em>In Indian economy the agriculture and allied sector contributes 13.7% of GDP and 27% of this is contributed by livestock sector alone. </em><em>In India as per NSSO, only about 5.1 percent of the households have some kind of access to modern technology in relation to animal husbandry activity. Estimates indicated that 60 per cent of farmers do not access any source of information for advanced agricultural technologies resulting in huge adoption gap. </em><em>Major ICT initiatives in India includes IFFCO Kisan Sanchar Limited,</em> <em>Village Knowledge Centres in Pondicherry, Warana Wired Village Project at (70 village in and around Warna) Maharashtra, KVK Babhaleshwar (MH), Gyandoot in Madhya Pradesh, e-VET Project in Madhya Pradesh, E-mail Conference System for Goat, Dairy Information and Services Kiosk, AKASHGANGA, Touch Screen Information Kiosk, Wireless Sensor Networks, Radio Frequency Identification. </em><em>ICT based livestock advisory services for knowledge dissemination to the farming communities for better and informed decision making at the farm level (Gulati et al., 2007). ICT helps in bridging the available communication gap. The enhanced and smooth communication results in the overall development of the livestock sector of the country.</em></p> S. J. Patel Rajesh Kumar G. M. Chaudhari S. W. Sawarkar S. S. Patil ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2014-06-01 2014-06-01 2 2 14 20 Genetic and Phenotypic Parameters of First Lactation Traits in Sahiwal Cattle: A Review http://ijvs.info/index.php/ijvs/article/view/35 <p><em>The heritability estimates of age at first calving (AFC), first lactation 305-day or less milk yield (FL305DMY) and first service period (FSP) of Sahiwal cattle ranged from zero to 0.80 ± 0.10, 0.04 ± 0.08 to 0.95 ± 0.24 and zero to 0.50 ± 0.18, respectively. Genetic correlation of AFC with FL305DMY and FSP ranged from -0.72 ± 0.22 to 0.44 and -0.83 ± 0.63 to 0.64 ± 0.001, respectively. However, genetic correlation of FL305DMY with FSP ranged from -0.90 ± 0.07 to 0.40 ± 0.36. The heritability estimates of the FSP was found to be low indicated the little scope for the genetic improvement; while higher heritability estimates of AFC and FL305DMY indicated the wide scope for further improvement in this traits through selection.</em></p> Vilas B. Dongre R. S. Gandhi T. V. Raja Anshu R. Ahlawat G. S. Sonawane ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2014-06-01 2014-06-01 2 2 21 27 Low Breeding Season and Estrus Synchronization in Buffalo (Bubalus bubalis) http://ijvs.info/index.php/ijvs/article/view/36 <p><em>Buffalo reproductive activity is characterized by a time period in which there is a lack or reduced reproductive activity (non- or low-breeding season or seasonal anestrus). The low breeding season is a major impediment in the improvement of reproductive as well as productive efficiency in buffalo. Factors affecting summer anestrus (major impediment in reproduction) include environment, nutrition and management. Unobserved (silent) estrus and an abbreviated duration of estrus are common during summer months. However, ovarian activity is not lacking in all animals during the low breeding season, since 64–75% females exhibit variable degrees of ovarian activity explained by Tailor et al (1990) and about 30% of cyclic animals show no estrus behavior during summer. Accordingly, some investigations have focused on protocols that are effective at inducing ovarian activity, stimulating estrous behavior and controlling ovulation during seasonal anestrus. </em></p> G. R. Bhat G. S. Dhaliwal S. P. S. Ghuman ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2014-06-01 2014-06-01 2 2 28 33 PCR-RFLP Analysis of Bovine Lactoferrin Promoter and its association with Mastitis Incidence in Crossbred (Karan Fries) Cattle http://ijvs.info/index.php/ijvs/article/view/37 <p><em>Present investigation was carried out with the aim to analyse the polymorphism of Lactoferrin promoter region, using PCR-RFLP with HaeIII and StyI restriction enzymes, in Karan Fries cattle (KF). A total of (n=150) KF animals were included in the study to know the distribution of different genotypes in the population. Hae III enzyme revealed two genotypes viz. EF and FF in Karan Fries cattle with genotype frequency as 14% and 86%. The allele frequencies of E and F allele in KF cattle were 0.07 and 0.93, respectively. </em><em>StyI restriction revealed three genotypes of lactoferrin gene promoter namely, AA, AB and BB in Karan Fries cattle having a frequency 18.67%, 76.66% and 4.67% respectively. The frequency of A and B allele in Karan Fries cattle was found to be 0.57 and 0.43 respectively. All the genotypes were studied for their association with Mastitis Incidence using Chi-Square Test, and it revealed a non-significant association at 5% level of significance.</em></p> Alka Chopra I. D. Gupta Archana Verma Vikas Vohra ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2014-06-01 2014-06-01 2 2 34 38 Effect of GnRH on Enhancement of Pregnancy Rates of Artificial Insemination in Cross-Bred Cattle http://ijvs.info/index.php/ijvs/article/view/38 <p><em>Though large number of cross-bred cattle are available, their productivity still remained marginal due to low conception rate and more repeat breeding problems with artificial insemination (AI). Therefore, the aim of the study was to evaluate the effect of supplementation of different doses of Gonadotropine Releasing Hormone (GnRH) along with AI and twelve days post AI in cross-bred dairy cattle on enhancement of pregnancy rates. Fifty five cross-bred dairy cattle that exhibited estrus and apparently healthy were randomly selected. The cattle were randomly divided into five equal groups, G1 (n=11) cattle were treated intramuscularly with each 20 µg GnRH, Buserelin acetate, at the time of AI. G2 (n=11) cattle have the same procedure as G1 but with the dose of 10µg Buserelin acetate. G3 (n=11) cattle were given the 10 µg GnRH (Buserelin acetate) twelve days post AI. The G4 (n=11) cows were given double insemination with 6-12 hours interval. The last group was given a single service which was considered as the control group. Pregnancy diagnosis was conducted after three months rectally. The pregnancy rates were 72.72%, 63.63%, 54.54%, 45.45% and 36.36 % in the cattle grouped with G1, G2, G3, G4 and G5, respectively. The pregnancy rates of the treated groups were higher than the control by 36%, 27%, 18% and 9 % from the first to the fourth group, respectively. The first two groups showed a significant statistical variation (P&lt;0.05).&nbsp; The other finding that was observed was that treated heifers became more responsive to the therapy than the treated cows with a significant variation (P&lt;0.05). The study concluded that the supplementation of GnRH at the time of AI can enhance pregnancy rate; and is helpful and cost effective to minimize financial losses incurred from milk production lost, managemental, and feed cost; by reducing the calving interval and treating repeat breeding cases.</em></p> K. G. Solmon Raju B. Sudhakara Reddy K. Prabhakar Rao ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2014-06-01 2014-06-01 2 2 39 44 Effect of Oocyte Collection Techniques, Maturation Media and BCB Staining on Buffalo Embryo Development in Vitro http://ijvs.info/index.php/ijvs/article/view/39 <p><em>The present experiment was conducted to know the effect of oocyte collection techniques, various maturation media and brilliant cresyl blue (BCB) stain on in vitro fertilization of buffalo oocytes. The rate of fertilization was comparatively lower in oocytes collected by aspiration than in slicing technique. Whereas, almost similar fertilization rate was observed in oocytes matured in TCM 199 + FBS + hCG media and Ham’s F-10 + FBS + hCG media. While, none of the oocytes matured either in TCM 199 alone or Ham’s F-10 alone was developed in to any stage of embryo. Further, better fertilization rate was obtained in BCB+ oocytes than BCB– oocytes group. The BCB+ oocytes collected by aspiration technique and matured in TCM 199 + FBS + hCG maturation media revealed 12.5% fertilization rate. BCB+ oocytes collected by slicing technique and matured in Ham’s F-10 + FBS + hCG media resulted in to 16.7% fertilization rate. BCB- oocytes collected by slicing technique and matured in Ham’s F-10 + FBS + hCG maturation media revealed 7.7% fertilization rate. </em></p> C. F. Chaudhari H. J. Derashri V. S. Dabas Gopal Puri J. H. Patel ##submission.copyrightStatement## http://creativecommons.org/licenses/by-nc-sa/4.0 2014-06-01 2014-06-01 2 2 45 49