Effect of Oocyte Collection Techniques, Maturation Media and BCB Staining on Buffalo Embryo Development in Vitro
Keywords:Aspiration, BCB, Ham’s F-10, Slicing, TCM 199
The present experiment was conducted to know the effect of oocyte collection techniques, various maturation media and brilliant cresyl blue (BCB) stain on in vitro fertilization of buffalo oocytes. The rate of fertilization was comparatively lower in oocytes collected by aspiration than in slicing technique. Whereas, almost similar fertilization rate was observed in oocytes matured in TCM 199 + FBS + hCG media and Ham’s F-10 + FBS + hCG media. While, none of the oocytes matured either in TCM 199 alone or Ham’s F-10 alone was developed in to any stage of embryo. Further, better fertilization rate was obtained in BCB+ oocytes than BCB– oocytes group. The BCB+ oocytes collected by aspiration technique and matured in TCM 199 + FBS + hCG maturation media revealed 12.5% fertilization rate. BCB+ oocytes collected by slicing technique and matured in Ham’s F-10 + FBS + hCG media resulted in to 16.7% fertilization rate. BCB- oocytes collected by slicing technique and matured in Ham’s F-10 + FBS + hCG maturation media revealed 7.7% fertilization rate.
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