PCR-RFLP Analysis of Bovine Lactoferrin Promoter and its association with Mastitis Incidence in Crossbred (Karan Fries) Cattle

  • Alka Chopra PhD (AGB), National Dairy Research Institute, Karnal (Haryana)
  • I. D. Gupta Principal Scientist, DCB Division, National Dairy Research Institute, Karnal (Haryana)
  • Archana Verma Principal Scientist, DCB Division, National Dairy Research Institute, Karnal (Haryana)
  • Vikas Vohra Senior Scientist, National Bureau of Animal Genetic Resources, Karnal (Haryana)
Keywords: Lactoferrin Gene, RFLP-PCR, Mastitis, Karan Fries

Abstract

Present investigation was carried out with the aim to analyse the polymorphism of Lactoferrin promoter region, using PCR-RFLP with HaeIII and StyI restriction enzymes, in Karan Fries cattle (KF). A total of (n=150) KF animals were included in the study to know the distribution of different genotypes in the population. Hae III enzyme revealed two genotypes viz. EF and FF in Karan Fries cattle with genotype frequency as 14% and 86%. The allele frequencies of E and F allele in KF cattle were 0.07 and 0.93, respectively. StyI restriction revealed three genotypes of lactoferrin gene promoter namely, AA, AB and BB in Karan Fries cattle having a frequency 18.67%, 76.66% and 4.67% respectively. The frequency of A and B allele in Karan Fries cattle was found to be 0.57 and 0.43 respectively. All the genotypes were studied for their association with Mastitis Incidence using Chi-Square Test, and it revealed a non-significant association at 5% level of significance.

References

1. Barkema H.W. (1998). Incidence of clinical mastitis in dairy herds grouped in three categories by bulk milk somatic cell counts. J Dairy Sci, 81 (2): 411-419
2. Daly M., Ross P., Giblin L. and Buckley F. (2006). Polymorphisms within the lactoferrin gene promoter in various cattle breeds. Anim Biotechnol 17:33–42. doi:10.1080/10495390500460965
3. Falconer D.S. and Mackay. (1996). Introduction to Quantitative Genetics 4th edition. Addison Wesley Longman Ltd. Essex, England.
4. Halloran F., Bahar B., Buckley F., O’Sullivan O., Sweeney T. and Giblin L. (2009). Characterisation of single nucleotide polymorphisms identified in the bovine lactoferrin gene sequences across a range of dairy cow breeds. Biochimie 91:68–75. doi:10.1016/j.biochi.2008.05.011
5. Hirvoen J., Eklund K. and Teppo A.M. (1999). Acute phase response in dairy cows with experimentally induced mastitis. Acta Vet Scand, 40: 35-46
6. Huang J., Wang H., Wang C., Li J., Li Q., Hou M. and Zhong J. (2010). Single nucleotide polymorphisms, haplotypes and combined genotypes of lactoferrin gene and their associations with mastitis in Chinese Holstein cattle. Mol Biol Rep. 2010 Jan; 37(1):477-83. doi: 10.1007/s11033-009-9669-1. Epub 2009 Aug 12.
7. Li G.H., Zhang Y., Sun D.X. and Li N. (2004). Study on the polymorphism of bovine lactoferrin gene and its relationship with mastitis. Anim Biotechnol 15:67–76. doi:10.1081/ABIO-120037899
8. Metz-Boutique M.H., Jolles J., Mazurier J., Schoentgen F., Legrand D., Spik G., Montreuil J. and Jolles P. (1984). Human lactotransferrin: amino acid sequence and structural comparisons with other transferrins. Europ. J. of Biochem., 145: 659:676
9. Sambrook J. and Russel D.W. (2001). Molecular Cloning : A laboratory manual. 3rd Ed. Vol 1. Cold Spring Harbor Laboratory press. New York, USA
10. Seyfert H.M., Tuckoricz A., Interthal H., Koczan D. and Hobom G. (1994). Structure of the bovine lactoferrin-encoding gene and its promoter. Gene 143:265–269. doi:10.1016/0378-1119(94)90108-2
Published
2014-06-01
How to Cite
Chopra, A., Gupta, I., Verma, A., & Vohra, V. (2014). PCR-RFLP Analysis of Bovine Lactoferrin Promoter and its association with Mastitis Incidence in Crossbred (Karan Fries) Cattle. The Indian Journal of Veterinary Science, 2(2), 34-38. Retrieved from http://ijvs.info/index.php/ijvs/article/view/37
Section
Articles